dld 1 cell viability assay compound cytotoxicity Search Results


dld 1  (ATCC)
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ATCC dld 1
Cell viability graphs of studied cell lines (A: <t>DLD‐1;</t> B: HL‐60 and C: HUVEC cell lines).
Dld 1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dld+1+cell+viability+assay+compound+cytotoxicity/DLD-1/pmc13670403-161-5-8
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cell lines sw480 atcc n a dld1 atcc n a hct116 atcc n a hek293t atcc n a experimental models  (ATCC)
99
ATCC cell lines sw480 atcc n a dld1 atcc n a hct116 atcc n a hek293t atcc n a experimental models
Figure 5. NEIL1 directly transcriptionally upregulates COL17A1 expression through forming a NEIL1/SATB2/c-Myc/POLR2A complex (A) CUT&Tag density heatmap of genes enriched by NEIL1 in <t>HCT116</t> cells stably expressing FLAG-NEIL1, within 3 kb around TSS (n = 2). (B) Heatmap of ATAC-seq performed in NEIL1-KO and control DLD-1 cells, within 3 kb around TSS (n = 2). (C) Venn diagram for the intersection of ATAC-seq (purple), RNA-seq (blue), CUT&Tag: NEIL1 (green), and CUT&Tag: SATB2 (orange) to screen out the potential genes directly regulated by both NEIL1 and SATB2 at the transcriptional level and associated with chromatin accessibility. (D and E) RT-qPCR (D) and western blot (E) of COL17A1 and ITGA1 expression in NEIL1-KO and control DLD-1 cells. (F) Schematic illustration of COL17A1 promoter and enhancer, which contains NEIL1-binding site (orange, 0 to 1,000) and SATB2-binding site (green, 7,500 to 8,500).
Cell Lines Sw480 Atcc N A Dld1 Atcc N A Hct116 Atcc N A Hek293t Atcc N A Experimental Models, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dld+1+cell+viability+assay+compound+cytotoxicity/SW480/pm38175757-217-176-179
Average 99 stars, based on 1 article reviews
cell lines sw480 atcc n a dld1 atcc n a hct116 atcc n a hek293t atcc n a experimental models - by Bioz Stars, 2026-10
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Image Search Results


Cell viability graphs of studied cell lines (A: DLD‐1; B: HL‐60 and C: HUVEC cell lines).

Journal: Chemistry & Biodiversity

Article Title: Biological Activities and Phytochemical Composition of Arum rupicola var. rupicola : An In Vitro and In Silico Study

doi: 10.1002/cbdv.71785

Figure Lengend Snippet: Cell viability graphs of studied cell lines (A: DLD‐1; B: HL‐60 and C: HUVEC cell lines).

Article Snippet: Cytotoxicity studies were conducted using DLD‐1 (colon carcinoma; ATCC CVCL_0248), HUVEC (normal endothelial; ATCC CCVL_2959), and HL‐60 (leukemia; ATCC CVCL_0002) cell lines.

Techniques:

Selectivity indices of the extracts against the cell lines.

Journal: Chemistry & Biodiversity

Article Title: Biological Activities and Phytochemical Composition of Arum rupicola var. rupicola : An In Vitro and In Silico Study

doi: 10.1002/cbdv.71785

Figure Lengend Snippet: Selectivity indices of the extracts against the cell lines.

Article Snippet: Cytotoxicity studies were conducted using DLD‐1 (colon carcinoma; ATCC CVCL_0248), HUVEC (normal endothelial; ATCC CCVL_2959), and HL‐60 (leukemia; ATCC CVCL_0002) cell lines.

Techniques:

Figure 5. NEIL1 directly transcriptionally upregulates COL17A1 expression through forming a NEIL1/SATB2/c-Myc/POLR2A complex (A) CUT&Tag density heatmap of genes enriched by NEIL1 in HCT116 cells stably expressing FLAG-NEIL1, within 3 kb around TSS (n = 2). (B) Heatmap of ATAC-seq performed in NEIL1-KO and control DLD-1 cells, within 3 kb around TSS (n = 2). (C) Venn diagram for the intersection of ATAC-seq (purple), RNA-seq (blue), CUT&Tag: NEIL1 (green), and CUT&Tag: SATB2 (orange) to screen out the potential genes directly regulated by both NEIL1 and SATB2 at the transcriptional level and associated with chromatin accessibility. (D and E) RT-qPCR (D) and western blot (E) of COL17A1 and ITGA1 expression in NEIL1-KO and control DLD-1 cells. (F) Schematic illustration of COL17A1 promoter and enhancer, which contains NEIL1-binding site (orange, 0 to 1,000) and SATB2-binding site (green, 7,500 to 8,500).

Journal: Cell reports

Article Title: NEIL1 drives the initiation of colorectal cancer through transcriptional regulation of COL17A1.

doi: 10.1016/j.celrep.2023.113654

Figure Lengend Snippet: Figure 5. NEIL1 directly transcriptionally upregulates COL17A1 expression through forming a NEIL1/SATB2/c-Myc/POLR2A complex (A) CUT&Tag density heatmap of genes enriched by NEIL1 in HCT116 cells stably expressing FLAG-NEIL1, within 3 kb around TSS (n = 2). (B) Heatmap of ATAC-seq performed in NEIL1-KO and control DLD-1 cells, within 3 kb around TSS (n = 2). (C) Venn diagram for the intersection of ATAC-seq (purple), RNA-seq (blue), CUT&Tag: NEIL1 (green), and CUT&Tag: SATB2 (orange) to screen out the potential genes directly regulated by both NEIL1 and SATB2 at the transcriptional level and associated with chromatin accessibility. (D and E) RT-qPCR (D) and western blot (E) of COL17A1 and ITGA1 expression in NEIL1-KO and control DLD-1 cells. (F) Schematic illustration of COL17A1 promoter and enhancer, which contains NEIL1-binding site (orange, 0 to 1,000) and SATB2-binding site (green, 7,500 to 8,500).

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays BCA Protein Assay Kit GLPBIO Cat# GK10009 CCK8 Assay Kit MCE Cat# HY-K0301 hCytokine Array Kit R&D Cat# ARY005B Human CCL2 ELISA Kit MultiSciences Cat# EK18 Human GM-CSF ELISA Kit MultiSciences Cat# EK163 Human CD54 ELISA Kit MultiSciences Cat# EK189 Human IL-8 ELISA Kit MultiSciences Cat# EK108 Human CXCL1 ELISA Kit MultiSciences Cat# EK196 LDH Cytotoxicity Assay Kit Abbkine Cat# KTA1030 Dual-Luciferase Reporter Assay System promega Cat# E1910 LightShift Chemiluminescent EMSA Kit Invitrogen Cat# 20158 TRIzol Reagent Invitrogen Cat# 15596018 PrimeScript RT Master Mix TAKARA Cat# RR036A iTaq Univer SYBR Green Supermix BIO-RAD Cat# 1725122 23Taq PCR Master Mix APExBIO Cat# K1034 NovoNGS CUT&Tag High-Sensitivity Kit NovoProtein Cat# N259-YH01-01B jetPRIME Transfection Reagent Polyplus Cat# 101000046 Multiplexed Immunohistochemistry Kit PANOVUE Cat# 10217100100 Mammalian Nuclear and Cytoplasmic Protein Extraction Kit TransGen Cat# DE201-01 Deposited data RNA-seq This manuscript NCBI Sequence Read Archive PRJNA942418 ATAC-seq This manuscript NCBI Sequence Read Archive PRJNA944723 CUT&Tag seq This manuscript NCBI Sequence Read Archive PRJNA944724, and PRJNA944725 MS analysis This manuscript ProteomeXchange PXD040878 Experimental models: Cell lines SW480 ATCC N/A DLD1 ATCC N/A HCT116 ATCC N/A HEK293T ATCC N/A Experimental models: Organisms/strains Mouse: wild-type C57L/6J mice Beijing Vital River Laboratory Aninmal Technology N/A Mouse: C57L/6J -ApcMin/+ mice the Model Animal Research Center of Nanjing University N/A Mouse: C57L/6J -neil1 / mice Cyagen Biosciences Inc N/A Mouse: C57L/6J -neil1flox/flox mice Cyagen Biosciences Inc N/A Mouse: C57L/6J -villin-Cre mice Cyagen Biosciences Inc N/A Mouse:C57L/6J-Lgr5-EGFP-IRES-CreERT2 mice the Model Animal Research Center of Nanjing University N/A Mouse: BALB/c nude mice Beijing Vital River Laboratory Aninmal Technology N/A Oligonucleotides Primers for mouse genotype identification TSINGKE See Table S11 Primers for PCR and RT-qPCR TSINGKE See Table S12 Sequence of sgRNA, siRNA and shRNA This manuscript See Table S13 Sequence of DNA-EMSA probe SYNBIO Technologies See Table S14 (Continued on next page) 16 Cell Reports 43, 113654, January 23, 2024

Techniques: Expressing, Stable Transfection, Control, RNA Sequencing, Quantitative RT-PCR, Western Blot, Binding Assay